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BACTERIOLOGICAL ETIOLOGY AND SOME FACTORS RELATED TO COMMUNITY PNEUMONIA IN VIETNAM NATIONAL CHILDREN'S HOSPITAL BY MULTI-PRIMER PCR TECHNIQUE
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Abstract
Objective: To find out the bacterial etiology and some related factors causing community pneumonia in children at the National Children's Hospital by multi-primer PCR technique. Subjects and research methods: A prospective, cross-sectional study on 257 patients diagnosed with community-acquired pneumonia treated at the International Department - National Children's Hospital from August 2019 to February 2020. Results: 73.2% of pneumonia cases were detected by multi-primer PCR. H. Influenzae was the most common cause (52.1%), followed by S. pneumoniae (33.9%) and M. Pneumonia (12.5%). 23.3% of the patients were positive for 2 types of bacteria and 1.2% was positive for 3 types of bacteria. The multiplex PCR technique has a sensitivity of 91.5% and a specificity of 32.3% compared to nasopharyngeal culture. The results of the multiplex PCR test were not affected by the patient's pre-hospital antibiotic use nor the duration of illness. Conclusion: Multiprimer PCR technique is a valuable method for diagnosing bacterial etiology in community-acquired pneumonia. In which, H. Influenzae, S. pneumoniae and M. Pneumonia are the most common bacteria
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Keywords
Community-acquired pneumonia, etiology, children.
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