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TO INVESTIGATE CHARACTERISTICS OF PATIENT USING NON-INVASIVE PRE-IMPLANTATION GENETIC TEST
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Abstract
Objectives: To investigate characteristics of patient using non-invasive pre-implantation genetic test. Subject and methods: This descriptive cross-sectional study was conducted on 14 volunteer couples with indication for PGT-A và NiPGT-A from March 2020 to November 2020 at the Military of Institute Clinical Embryology and Histology, embryos were cultured by using single-drop culture process. Results: The mean age of 14 patients was 35.71 ± 3.17, Infertility 2 accounts for 85.71%, The main indications of PGT-A, NiPGT-A are consecutive implantation failure (85.71%) and advanced maternal age (57.14%), the mean level of AMH was 2.32ng/mL, the mean number of Antral follicles count was 12.07 ± 4.12, Total FSH dose used in controlled ovarian stimulation cycle was 2578.57 ± 483.66 IU, the mean duration of FSH administration was 10.14 ± 1.1 days, the average number of aspirated oocyte corona complexes was 8.5 ± 4.55 complexes. The mean number of oocytes after denudation was 8.07 ± 3.87. MII oocyte was 7.14 ± 3.63. The mean 2PN zygote was 5.21 ± 2.94. The mean MII oocyte rate was 89.84 ± 14.73%. The fertilization rate was 73 ± 18%, the percentage of day-3 cleavage embryo was 98.41 ± 4.03%, the blastulation rate was 58.08 ± 27.8%. Conclusions: The main indications for PGT-A, NiPGT-A are consecutive implantation failures (85.71%) and advanced maternal age (57.14%), the percentage of day-3 cleavage embryo was 98.41 ± 4.03%, the blastulation rate was 58.08 ± 27.8%.
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Keywords
single-drop embryo culture, individual embryo culture, in vitro fertilization, niPGT, noninvasive pre-implantation genetic testing.
References
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