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CONCENTRATION OF DNA AMPLIFICATION PRODUCT IN SPENT EMBRYO CULTURE MEDIA FOR NON-INVASIVE PREIMPLANTATION GENETIC TESTING
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Abstract
Objective: To evaluate the DNA concentration in spent culture media after whole genome amplification and some related factors in the noninvasive pre-implantation genetic test for aneuploidy. Materials and Methods: cross-sectional study of 31 blastocysts cultured in a single drop culture from day 3, volume 15 µL, assisted hatched on day three, collected culture sample on the day of full blastocyst formation, amplified the whole genome with the IonSingleseq kit kit, DNA concentration evaluation using Qbit hsDNA.Results: in our study, the amplification rate of culture media sample was 28/31 samples (90.32%), trophectoderm biopsy sample was 30/31 embryos (96.77%). The concentration of the DNA product in the post-amplified in culture media sample was 15.37 ± 8.75ng/µL. The DNA concentration of the trophectoderm biopsy sample was 18.78 ± 5.50ng/µL, the difference between the invasive and non-invasive groups was not statistically significant. There is no statistically relationship between embryo morphology and genome amplification results. DNA concentration of SCM samples of day 6 embryo was higher than day 5 embryo.Conclusion: The successful amplification rateof SCM samples is 90.32%, there is no statistically difference in concentration of DNA products between SCM and TE samples; The blastocyst day affects the DNA concentration of the whole genome amplification product in the SCM sample.
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Keywords
niPGT-A, SCM, embryo culture, non-invasive, pre-implantation genetic test, NGS
References
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