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Date Published: 07/12/2023
Abstract Views: 78
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DOI: https://doi.org/10.51298/vmj.v532i1B.7505
Issue
Vol. 532 No. 1B (2023)
Section
Các bài báo
How to Cite
Nguyễn, Đức Điển, Nguyễn, M. P., & Nguyễn, V. C. (2023). THE EXPERIMENTAL DETERMINATION OF LATERAL FLOW IMMUNOASSAYS SPECIFICATIONS TO DETECT AFLATOXIN M1 IN MILK. Vietnam Medical Journal, 532(1B). https://doi.org/10.51298/vmj.v532i1B.7505

THE EXPERIMENTAL DETERMINATION OF LATERAL FLOW IMMUNOASSAYS SPECIFICATIONS TO DETECT AFLATOXIN M1 IN MILK

Đức Điển Nguyễn1,, Minh Phương Nguyễn1, Văn Chuyên Nguyễn1
1 Military Medical University

Main Article Content

Abstract

Objective: Objective: Determine specifications of of lateral flow immunoassays to detect Aflatoxin M1. Methods: The study was conducted from November 2021 to June 2023. The study was conducted at the Department of Military Hygiene, Military Medical Academy. Determine specifications of of lateral flow immunoassays to detect Aflatoxin M1, including detection limits, cross-reactivity, stability, sensitivity, specificity, and compare the consistency of the kit and HPLC test results. Results: The lateral flow test strip cannot detect antigen concentrations below 2 ng/ml (negative result). At an antigen concentration of 2 ng/ml, the lateral flow test strip gave positive results in 4/5 tests. The lateral flow test strip did not cross-react with standard samples of Aflatoxin B1, Ochratoxin A, and Patulin at the concentrations tested. The sensitivity of the rapid test strip is 90% (18/20 samples), while the specificity is only 85% (17/20 samples). When evaluated on food samples, the test results of the kit are quite consistent (KAPPA = 0.63). Conclusion: Evaluation of the lateral flow test strip specifications for Aflatoxin M1 detection shows that the lateral flow test strip has sensitivity and specificity at a high level.

Article Details

Keywords

Lateral Flow Immunoassay; rapid detection; Aflatoxin M1

References

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