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ANTI-INFLAMMATORY, ANTI-PROTOZOAL ACTIVITIES OF SCOPARIA DULCIS
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Abstract
This study was designed to evaluate the anti-inflammatory and antiprotozoal activities of 30% and 96% ethanolic extracts and isolated compounds from the whole plant of Scoparia dulcis collected in Vinh Long. The results showed that 96% ethanolic extract inhibited nitric oxide production at concentrations below 100 μg/ml in the LPS-induced RAW 264.7 macrophages. In the model of carrageenan-induced paw edema in mice, the group treated with 96% ethanolic extract at a dose of 500 mg/kg indicated lower swelling (not statistically significant) than the negative control group at all time points of the experiment. The group treated with 30% ethanolic extract at a dose of 500 mg/kg showed lower swelling (statistically significant) compared with the negative control group at time point of 1h, 5h and 24h. Compared with the positive control group (treated with diclofenac at a dose of 5mg/kg), mice treated with 30% ethanolic extract, at a dose of 500 mg/kg showed no statistically significant difference in swelling compared with the positive control group in time point of 1h, 3h and 5h. The above results proved that the 30% ethanolic extract possessed a better anti-inflammatory effect than the 96% ethanolic extract, after 24 hours of experiment. In addition, 30% and 96% ethanolic extracts had antiprotozoal activity against Tetrahymena pyriformis after 24 hours of incubation with two these extracts at a concentration of 25 mg/ml. Using chromatographic techniques, 5 compounds were isolated and structurally determined from extract with 96% alcohol of Scoparia dulcis whole plant (cpd.1-5). Evaluating the antiprotozoal activity of compounds 1-3 indicated that all possessed moderate antiprotozoal activity.
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References
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