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EFFECT OF CYTOPLAMIC GRANULATION ON IN VITRO FERTILIZATION OUTCOMES
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Abstract
Objectives: The study aims to analyze the effects of centrally located cytoplasmic granular (CLCG) cytoplasm oocytes on fertilization rates, day 2 embryo rates and live birth outcomes in cycles. Methods: A prospective study conducted on 231 ICSI cycles at Andrology and Fertility Hospital of Hanoi from 2016 to 2019. All cycles used sperm ejaculated on the day of oocyte aspiration, and sperm quality meets WHO 2010 standards with a minimum normal shape ratio of 1%. Fertilization rates and day 2 embryo rates were compared between four groups of CLCG oocytes including: having only one abnormality, having at least one abnormality, having only two abnormalities and having at least two abnormalities. Live birth rates were investigated in 150 cycles with maternal age under 35 and oocyte number less than 15. Results: The fertilization rates of the group with at least one abnormality (72,1%), the group with at least two abnormalities (69,5%), and the group with only two abnormalities (70,3%) were statistically significant lower compared to the groups without the same characteristics were 79,0%, 81,7% and 80,8%, respectively (p < 0,05). The good day-2 embryo rates of the group with at least one abnormality (53,8%), the group with at least two abnormalities (57,3%) and the group with only two abnormalities (49,5%) were significantly lower than that of the groups without the same characteristics (65,8%; 76,5%; and 75,1%, respectively (p< 0,05)), The live-birth rates between cycles with CLCG and without CLCG had no statistically significant difference (57,6% vs. 44,0%, p>0,05). Conclusion: CLCG is an abnormal oocyte characteristic that might have negative effects on fertilization rates and day 2 embryo rates. However, the impact of CLCG oocytes on the live-birth rates in young patients who have few oocytes has not been determined.
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Keywords
: oocyte cytoplasm abnormalities, central granular cytoplasm, fertilization rates, day 2 embryo rates, live-birth rates.
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