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EVALUATE THE QUALITY OF ONE-STEP RT-qPCR HBV KIT TO DETECT THE SERUM pgRNA LEVEL IN CHRONIC HBV-INFECTED PATIENTS
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Abstract
Objective:This study was to evaluate the quality of one-step RT-qPCR HBV kit to detect the serum pgRNA level in chronic HBV-infected patients. Materials and methods: The limit of detection, the limit of quantitation, linear range, repeatability, precision, and specificity of one-step R&D RT-qPCR HBV kit were included in the study, as well as compared the capability with two-step RT-qPCR method to detect serum pgRNA level. Results: The limit of detection and the limit of quantitation were 70 copies/ml and 140 copies/ml of serum, respectively. The linear range was from 102 to 108 copies/ml, R2 = 0,996. The repeatability and precision of one-step R&D RT-qPCR HBV kit were in good performan with CV ≤ 0,03, deltaCt <0,5, and specificity of 100%. There was a high correlation in quantification of HBV-pgRNA between the two kits (R2= 0,9885). Conclusion: The one-step R&D RT-qPCR HBV kit could be useful for detection of serum pgRNA level and follow-up management of treated chronic HBV-infected patients.
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Keywords
Pregenomic RNA, one-step R&D RT-qPCR HBV kit, CHB
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