NGHIÊN CỨU ĐẶC ĐIỂM HOẠT TÍNH CHẾ TIẾT VÀ BIỂU LỘ MỘT SỐ THỤ THỂ CÓ LIÊN QUAN CỦA TẾ BÀO NK MÁU NGOẠI VI TRÊN BỆNH NHÂN UNG THƯ TUYẾN TIỀN LIỆT

Abstract

Objective: To evaluate the characteristics of NK cell activity and the expression of some activating and inhibitory receptors of peripheral blood NK cells in prostate cancer patients. Subjects and methods: The study was conducted on 30 patients diagnosed with prostate cancer at K Hospital - Tan Trieu campus. To analyze the NK cell activity, we used the NK-VUE kit (with IVD standard) to measure the ability of peripheral blood NK cells to release the cytokine Inteferon-gamma (IFNγ). To assess the expression level of the activating receptor (NKG2D) and inhibitory receptor (NKG2A), we used flow cytometry techniques to evaluate the expression level of these two receptors on NK cell populations in peripheral blood mononuclear cells (PBMCs) of patients. We clarify the correlation among the above indicators. Result: The average levels of IFNγ secreted by NK cells (NKA-IFNγ) in the prostate cancer patient group is 340.17 pg/mL; 80% (24 cases) of patients have NKA-IFNγ ≤ 500 (pg/mL); and 70% (21 cases) have NKA-IFNγ ≤ 200 (pg/mL). The expression levels of NKG2D and NKG2A in NK cells were 76.46% and 19.43%, respectively. Conclusions: In patients with prostate cancer, the secretory activity of NK cells was reduced. There is a negative correlation between the level of NKG2A inhibitory receptor expression and NK cell activity.