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THE CORRELATION BETWEEN THE SECRETORY ACTIVITY OF PERIPHERAL BLOOD NK CELLS AND THE CYTOTOXIC CAPACITY OF EX-VIVO EXPANDED NK CELLS DERIVED FROM PROSTATE CANCER PATIENTS
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Abstract
Objective: To clarify the correlation between the secretory activity of NK cells derived from the peripheral blood of prostate cancer patients and the target cell killing capacity of NK cells after ex-vivo expansion. Research objects and methods: The study collected data on the peripheral blood NK cell secretory activity of 14 prostate cancer patients and the culturing of NK cells from the above patients within 14 days. We evaluated the killing ability of prostate cancer (PC3) cells from freshly isolated NK cells (day D0) and cells after proliferation culture (day D14) using flow cytometry. Results: The ability to kill target cells increased from 14.13% on day 0 to 51.12% on day 14. In particular, the amount of NKA-IFNγ released from NK cells in prostate cancer patients strongly correlates to how well post-proliferative NK cells kill PC3 cells (r = 0.699, p = 0.005). Conclusion: In prostate cancer patients, the secretory activity of peripheral blood NK cells showed a strong correlation with the cytotoxic capacity of the NK cells after expansion.
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