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IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITIES OF STEM BARK EXTRACT OF OROXYLUM INDICUM ON CARBON TETRACHLORIDE-INDUCED LIVER DAMAGE IN MICE
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Abstract
The present study aimed to evaluate the acute toxicity, the in vitro antioxidant capacity and in vivo hepatoprotective effect of the extract from Oroxylum indicum stem bark. Concerning the DPPH free-radical scavenging assay, the extract demonstrated an active antioxidant activity with an IC50 of 69.5 µg/mL. In the acute toxicity experiment, where a dose of 10 g/kg was administered, all treated mice remained alive and showed no signs of poisoning after 14 days of receiving the extract. In a model of CCl4-induced liver injury, pretreatment of the mice with the extract at doses of 900 mg/kg and 1200 mg/kg significantly reduced the increase in AST and ALT levels compared to the toxic group at all administered doses. The results of histopathological study were consistent with the biochemical parameters.
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