KHẢO SÁT CÁC PHƯƠNG PHÁP XÉT NGHIỆM VI SINH Ở BỆNH NHÂN GHÉP GIÁC MẠC ĐƯỢC CHIẾU TIA GAMMA

Abstract

Purpose: To evaluate microbiological tests in therapeutic penetrating keratoplasty using gamma irradiated corneal tissues. Methods: Cross-sectional, observational study, performed on 33 penetrating keratoplasty patients using gamma irradiated corneal graft from 11/2018 to 08/2023 at Ho Chi Minh City Eye Hospital. Microbiological tests included smear, culture, multiplex polymerase chain reaction (PCR), and pathology. Results: There were 3 groups of pathogens including fungi (22 eyes), bacteria (6 eyes), and herpes simplex virus type 1 (HSV-1, 5 eyes) causing keratitis. Among them, one eye was infected by microsporidia, which was grouped with fungi. Smear had results after 30 minutes; culture result on average after 3.0 ± 1.0 days (ranging from 2 to 7 days); PCR results after 1.6 ± 0.6 days (ranging from 1 to 3 days); and pathology after 8.5 ± 3.3 days (ranging from 5 to 15 days). The difference in results time was statistically significant (p < 0.0001). Fungi were diagnosed mainly based on smear and pathology, with rates of 76.2% and 66.7%, respectively. In particular, microsporidia were diagnosed based on PCR. Bacteria were diagnosed mainly based on clinical characteristics with a rate of 100.0%. HSV-1 were diagnosed by clinical obsertvation with a rate of 60.0%; and 40.0% by PCR. Conclusion: Microbiological tests were effective methods to diagnose and identify pathogens, optimizing the effectiveness of therapeutic penetrating keratoplasty in the treatment of infectious keratitis.