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ASSOCIATION BETWEEN OF METALLO-Β-LACTAMASE NDM, IMP, VIM GENOTYPES AND CARBAPENEM ANTIBIOTIC RESISTANCE SOME OF GRAM-NEGATIVE BACTERIA FROM PNEUMONIA PATIENTS AT CAN THO CITY GENERAL HOSPITAL IN 2022-2023
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Abstract
Background: In recent years, there has been an increase in the number of gram-negative bacilli that produce metallo-β-lactamase (MBL) with NDM, IMP, and VIM genes, resulting in increased resistance to carbapenem antibiotics among pneumonia patients. Objective: Determine the genotype rate and association between of metallo - β - lactamase NDM, IMP, and VIM genotypes with carbapenem antibiotic resistance of gram-negative bacilli Escherichia coli, Klebsiella pneumonia, Acinetobacter baumannii, and Pseudomonas aeruginosa in pneumonia patients at Can Tho City General Hospital in 2022-2023. Materials and methods: a cross-sectional descriptive study on 92 gram-negative bacilli in pneumonia patients. Results: In the study group of gram-negative bacilli, NDM genotype accounted for 96.8%, IMP genotype accounted for 32.3%, and VIM genotype accounted for 1.6%. The prevalence rates of Klebsiella pneumoniae carrying the NDM gene were 63.6%, 6.1% for NDM+IMP genes, and 3.0% for NDM+IMP+VIM genes. The rates of single NDM gene and two NDM+IMP genes combination in Escherichia coli were 15.0% and 5.0%, respectively. In Acinebacter baumannii, the rates of single gene NDM, single gene IMP, and the combination of two NDM+IMP genes were 40.0%, 7.1%, and 25.0%, respectively. For Pseudomonas aeruginosa, the rates of the single NDM gene combination and the two NDM+IMP gene combination were 36.4% and 63.6%, respectively. We observed a significant association between carbapenem resistance and NDM genotype in gram-negative bacilli (p<0.001), with an OR=22.56 (95% CI: 4.68 - 108.76). Conclusions: In a study group, the distribution of gene combinations for each type of gram-negative bacilli was examined. One strain of Klebsiella pneumoniae was found to carry a combination of three genes. The NDM genotype and NDM monogene combination were found to be associated with carbapenem resistance (p < 0.05).
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Keywords
pneumonia, gram-negative bacteria, metallo-β-lactamase NDM, IMP,VIM, carbapenem antibiotic resistance.
References
2. Trần Huy Hoàng, cộng sự (2016), "Sự xuất hiện vi khuẩn đường ruột mang gen NDM-1 phân lập trên bệnh nhân điều trị tại Bệnh viện Thanh Nhàn, Hà Nội giai đoạn 2010-2014", Tạp chí Y học dự phòng, 10(183), tr.78-85.
3. Boukaré Kaboré, Henri S.Ouédraogo, Oumarou Zongo, et al. (2023), "Emergence of New Delhi Metallo - β - lactamase (NDM) genes detected from clinical strains of Escherichia coli isolated in Ouagadougou, Burkina Faso", International journal of microbiology, pp.679 - 687.
4. E.boyd S., M.Livermore D., C.Hopper D., et al. (2020), "Metallo-β-Lactamases: structure, function, epidemiology, treatment options, and the development pipeline", Antimicrob Agents Chemother,, 6 (10), pp.21-40, https://doi.org/10.1128/AAC.00397-20.
5. Exner M., Bhattacharya S., Christiansen B. G. J., et al. (2017), "Antibiotic resistance: What is so special about multidrugresistant Gram - negative bacteria", GMS Hygiene and Infection Control, 12, pp 1 - 24.
6. Kumari M., Verma S., Venkatesh V., et al. (2021), "Emergence of blaNDM-1 and blaVIM producing gram-negative bacilli in ventilator-associated pneumonia at AMR Surveillance Regional Reference Laboratory in India", PLoS One, 16(9), pp.256-308, https://doi.org/ 10.1371/journal.pone.0256308.
7. Mohamed Amira, Daef Enas, Nafie Amany, et al. (2021), "Characteristics of carbapenem-resistant gram-negative bacilli in patients with ventilator-associated pneumonia", Antibiotics, 10(11), pp.1325-1335, https://doi.org/10.3390/ antibiotics10111325.
8. Truong Thien Phu, Truong Ngoc Huong, Le Phuong Mai, et al. (2020), "Carbapenem resistant gene of Pseudomonas aeruginosa", Biomedical Journal of Scientific & Technical Research, 29(1), pp.22055-22057, DOI: 10.26717/BJSTR.2020.29.004730.