SỬ DỤNG PHƯƠNG PHÁP ĐO QUANG ĐỂ XÁC ĐỊNH GIÁ TRỊ ẤN ĐỊNH CỦA MẪU SINH PHẨM HUYẾT HỌC TRONG KIỂM TRA CHẤT LƯỢNG XÉT NGHIỆM HUYẾT HỌC

Abstract

Researching the stability of blood cell counts (human red blood cells, pseudo-leucocytes, pseudo-platelets) used to implement an external quality assessment (EQA) to check the quality of hematology tests are necessary for hematology laboratories in Vietnam. In this research, the research team determined the assessment value of blood cell count (human RBCs, pseudo-leucocytes, and pseudo-platelets) by the optical method used in hematology EQA programs. This Hematological reference sample is controlled at 3 levels of low, normal and high concentrations. Determine the assigned value (mean ± 2SD) of the hematology analyzer line by photometric method on the Celldyn 3200, Celldyn Ruby, samples will be sent to 10 different laboratories using the Celldyn 3200 series, Celldyn Ruby for review. Research results have determined the assigned value of the Celldyn 3200 and Celldyn Ruby series at 3 concentration levels: the Celldyn 3200 series has 80%, the Celldyn Ruby has 90% of laboratories with analytical results within the price range. fixed value. The results of measuring the number of human RBCs, pseudo-leucocytes, and pseudo-platelets on each analytical device among 10 laboratories are equivalent, with the same concentration level, the Celldyn 3200 and Celldyn Ruby models will give simulated white blood cell results. and red blood cells are similar, only the simulated platelet index is different and this difference is statistically significant (p < 0.05). Thus, hematological reference samples measuring the number of blood cell meet the standards so that they can evaluate the results of laboratories using the optical method: Celldyn 3200, Celldyn Ruby.