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DETECTION OF CARBAPENEMASE ENCODING GENES IN PEUDOMONAS AERUGINOSA ISOLATES WITH PHENOTYPIC NON-RESISTANT PROFILE
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Abstract
Objectives: The escalating prevalence of multidrug-resistant Pseudomonas aeruginosa presents a formidable challenge in effectively treating infections attributed to this bacterium. This research aimed to detect carbapenemase encoding genes within P. aeruginosa strains that manifest non-resistance to conventional antibiotics. Materials and methods: In this cross-sectional study, 24 P. aeruginosa strains displaying non-resistance to the tested antibiotics were examined. Multiplex Real-time PCR was employed to identify specific carbapenemase encoding genes within these strains. Results: Out of the 24 tested P. aeruginosa strains, four were identified as carriers of carbapenemase genes. Notably, two strains harboured the blaKPC gene, one carried the blaNDM gene, and another carried the blaOXA-48 gene. Conclusion: The combination of bacterial drug resistance phenotypes and genotypes is imperative for the comprehensive management and surveillance of antibiotic resistance in P. aeruginosa.
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Keywords
P. aeruginosa, carbapenemase genes, Hai Phong International General Hospital.
References
2. Eun-Jeong Yoon and Seok Hoon Jeong*, “Mobile Carbapenemase Genes in Pseudomonas aeruginosa”, Frontiers in Microbiology, 2021.
3. “Bộ Y tế (2017). Hướng dẫn thực hành kỹ thuật xét nghiệm Vi sinh lâm, Nhà xuất bản Y học, Hà Nội”.
4. Caterina Aurilio, et al, “Mechanisms of Action of Carbapenem Resistance”, Antibiotics, vol 11, số p.h 421, 2022.
5. Saeed Falahat et al, “Detection of KPC Carbapenemase in Pseudomonas aeruginosa Isolated From Clinical Samples Using Modified Hodge Test and Boronic Acid Methods and Their Comparison With the Polymerase Chain Reaction”, Jundishapur J Microbiol, vol 9, số p.h 9, 2016.
6. Jovcic, B., Lepsanovic, Z., Suljagic, V., Rackov, G., Begovic, J., Topisirovic, L., et al, “Emergence of NDM-1 metallo-beta lactamase in Pseudomonas aeruginosa clinical isolates from Serbia”, Antimicrob. Agents Chemother, vol 55, tr 3929–3931, 2011.
7. Khanda Abdulateef Anoar et al, “Detection of Metallo-Beta-lactamase enzyme in some Gram negative bacteria isolated from burn patiens in Sulaimani city, Iraq.”, European Scientific Journal, vol 10, số p.h 3, tr 1857–7881, 2014.
8. Doha Omer Ali, Mohamed M.A. Nagla, “Molecular Detection of bla OXA-48 Gene Encoding Carbapenem Resistance Pseudomonas aeruginosa Clinical Isolates from Khartoum State Hospitals, Sudan”, The preprint server for health science, 2020.
9. Mudathir Abdallah Adam, Wafa I. Elhag, “Prevalence of metallo-β-lactamase acquired genes among carbapenems susceptible and resistant Gram-negative clinical isolates using multiplex PCR, Khartoum hospitals, Khartoum Sudan”, BMC Infectious Diseases, vol 18, 2018.
10. Florence Depardieu et al., “Modes and Modulations of Antibiotic Resistance Gene Expression”, American Society for Microbiology, vol 20, số p.h 1, 2007.