NGHIÊN CỨU ĐỘ ỔN ĐỊNH ĐỒNG THỜI CỦA GLUCOSE HUYẾT TƯƠNG VÀ HBA1C TRONG MÁU TOÀN PHẦN Ở CÁC ĐIỀU KIỆN NHIỆT ĐỘ KHÁC NHAU

Abstract

Objective: To study the simultaneous stability of human plasma glucose and whole blood HbA1c at different storage temperatures. Materials and methods: 10 ml of whole blood from 10 volunteers was collected into anticoagulation tubes with K2EDTA. A portion of whole blood was divided into sterile test tubes to quantify HbA1c, and the remaining portion was centrifuged to separate the plasma for glucose quantification. Whole blood and plasma samples are stored at 3 different temperature conditions. At the time of the investigation, HbA1c concentrations in whole blood samples were quantified by immunophotometric turbidimetric method, while plasma glucose concentrations were measured by enzymatic photometric method. Results: The relationship between plasma glucose and HbA1c at T₀ was not found. HbA1c was stable at 20-25 °C for less than 24 hours, at 2-8 °C for less than 1 day and at -20 °C for less than 30 days. Plasma glucose was stable at 20-25 °C for less than 24 hours, at 2-8 °C for less than 2 days and at -20°C for less than 3 months. Conclusion: Plasma glucose was considered more stable than whole blood HbA1c under the same storage conditions