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INVESTIGATIONS OF ACETYLCHOLINESTERASE INHIBITORY ACTIVITY OF SEVERAL FERNS AND TRADITIONAL MEDICINE FORMULAS
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Abstract
Objective: Alzheimer’s disease (AD) is a degenerative neurological disease that is common in the elderly. The acetylcholinesterase (AChE) inhibitors, which help maintain acetylcholine content in synaptic clefts, are the first choice in treating AD’s symptoms. Herbs and traditional medicine formulas (TMF) have shown the potential in inhibiting AChE in vitro. This study aimed to screen the AChE inhibitory activity in vitro of some ferns and TMF. Materials and Methods: In this study, we screened the AChE inhibitory effect in vitro of 5 ferns and 6 TMF. The herbs were extracted with ethanol 70% whereas TMF were decocted with clean water. Subsequently, AChE inhibitory effect was screened using the Ellman’s colorimetric method. Results: The extracts of Acrostichum aureum L., Ceratopteris thalictroides (L.) Brongn, Quy Ty Thang, and Bao Hoa Hoan Gia Giam exhibited the highest AChE inhibitory effect, with IC50 values of 60.8 ± 0.49 μg/mL, 132.6 ± 0.45 μg/mL, 263.8 ± 14 μg/mL, and 124.2 ± 12.9 μg/mL, respectively. Conclusion: The study suggests potential AChE inhibition, opening the foundation for exploring and applying medicinal herbs, especially fern species and TMF in AD treatment.
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Keywords
AChE inhibition, in vitro, Ellman, ferns, traditional medicine formulas
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