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Date Published: 10/06/2024
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DOI: https://doi.org/10.51298/vmj.v539i2.9874
Issue
Vol. 539 No. 2 (2024)
Section
Các bài báo
How to Cite
Lưu, T. N., Nguyễn, V. A., Lê, N. M. H., Chu, D. S., Lê, T. T. N., & Lê, H. L. H. (2024). DETECTION OF EXTENDED-SPECTRUM β-LACTAMASE GENES IN PSEUDOMONAS AERUGINOSA ISOLATES WITH PHENOTYPIC NON-RESISTANT PROFILE. Vietnam Medical Journal, 539(2). https://doi.org/10.51298/vmj.v539i2.9874

DETECTION OF EXTENDED-SPECTRUM β-LACTAMASE GENES IN PSEUDOMONAS AERUGINOSA ISOLATES WITH PHENOTYPIC NON-RESISTANT PROFILE

Thị Nga Lưu, Văn An Nguyễn, Nguyễn Minh Hoa Lê, Dũng Sĩ Chu, Thị Trang Nhung Lê, Hạ Long Hải Lê

Main Article Content

Abstract

Objectives: Pseudomonas aeruginosa is a leading cause of infections in most parts of the world. The production of extended-spectrum β-lactamase (ESBL) is an essential mechanism of β-lactam resistance in the bacterium. Methods: In this cross-sectional study, 24 P. aeruginosa strains displaying non-resistance to the tested antibiotics were examined. Multiplex Real-time PCR was employed to identify ESBL genes within these strains. Results: Of the 24 Pseudomonas aeruginosa isolates with a phenotypic non-resistant profile, 17 (70.8%) isolates were found to contain ESBL genes. Among these ESBL-positive isolates, the blaCTX-M (82.4%) was the most common gene, followed by blaTEM (35.3%), and blaSHV (23.5%), either alone or in combination. Conclusion: The results of this study showed the notable prevalence of ESBL genes among the clinical isolates of P. aeruginosa, indicating the urgency for the implementation of appropriate follow-up measures for infection control and proper administration of antimicrobial agents in medical settings

Article Details

Keywords

Psedomonas aeruginosa, ESBL gene, Real- time PCR

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