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Date Published: 10/06/2024
Abstract Views: 53
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DOI: https://doi.org/10.51298/vmj.v539i2.9874
Issue
Vol. 539 No. 2 (2024)
Section
Các bài báo
How to Cite
Lưu, T. N., Nguyễn, V. A., Lê, N. M. H., Chu, D. S., Lê, T. T. N., & Lê, H. L. H. (2024). DETECTION OF EXTENDED-SPECTRUM β-LACTAMASE GENES IN PSEUDOMONAS AERUGINOSA ISOLATES WITH PHENOTYPIC NON-RESISTANT PROFILE. Vietnam Medical Journal, 539(2). https://doi.org/10.51298/vmj.v539i2.9874

DETECTION OF EXTENDED-SPECTRUM β-LACTAMASE GENES IN PSEUDOMONAS AERUGINOSA ISOLATES WITH PHENOTYPIC NON-RESISTANT PROFILE

Thị Nga Lưu, Văn An Nguyễn, Nguyễn Minh Hoa Lê, Dũng Sĩ Chu, Thị Trang Nhung Lê, Hạ Long Hải Lê

Main Article Content

Abstract

Objectives: Pseudomonas aeruginosa is a leading cause of infections in most parts of the world. The production of extended-spectrum β-lactamase (ESBL) is an essential mechanism of β-lactam resistance in the bacterium. Methods: In this cross-sectional study, 24 P. aeruginosa strains displaying non-resistance to the tested antibiotics were examined. Multiplex Real-time PCR was employed to identify ESBL genes within these strains. Results: Of the 24 Pseudomonas aeruginosa isolates with a phenotypic non-resistant profile, 17 (70.8%) isolates were found to contain ESBL genes. Among these ESBL-positive isolates, the blaCTX-M (82.4%) was the most common gene, followed by blaTEM (35.3%), and blaSHV (23.5%), either alone or in combination. Conclusion: The results of this study showed the notable prevalence of ESBL genes among the clinical isolates of P. aeruginosa, indicating the urgency for the implementation of appropriate follow-up measures for infection control and proper administration of antimicrobial agents in medical settings

Article Details

Keywords

Psedomonas aeruginosa, ESBL gene, Real- time PCR

References

1. Bộ Y Tế (2023). Báo Cáo Giám Sát Kháng Kháng Sinh Tại Việt Nam Năm 2020 Của Bộ Y Tế, công bố tháng 11/2023.
2. Bộ Y tế (2017). Hướng dẫn thực hành kỹ thuật xét nghiệm Vi sinh lâm sàng, Nhà xuất bản y học, Hà Nội.
3. A. Peymani et al (2017). Distribution of blaTEM, blaSHV, and blaCTX-M genes among ESBL-producing P.aeruginosaisolated from Qazvin and Tehran hospitals, Iran. Journal of Preventive Medicine and Hygiene. 58: p. 155-160.
4. Hayford Odoi et al (2021). Prevalence and Phenotypic and Genotypic Resistance Mechanisms of Multidrug-Resistant Pseudomonas aeruginosa Strains Isolated from Clinical, Environmental, and Poultry Litter Samples from the Ashanti Region of Ghana. Journal of Environmental and Public Health.
5. K. S. M. Azab et al (2021). Distribution of Extended-Spectrum β-Lactamase (ESBL)-Encoding Genes among Multidrug-Resistant Gram-Negative Pathogens Collected from Three Different Countries. Antibiotics. 10(3): p.247.
6. Mojisola C. Hosu et al (2021). Detection of extended spectrum beta‑lactamase genes in Pseudomonas aeruginosa isolated from patients in rural Eastern Cape Province, South Africa. Scientific Reports. 11(1): p. 7100.
7. M. Castanheira et al(2021). Extended-spectrum β -lactamases: an update on their characteristics, epidemiology and detection. JAC-Antimicrobial Resistance. 3(3): p.54-58.
8. Nicole Roschanski (2014). Development of a Multiplex Real-Time PCR for the Rapid Detection of the Predominant Beta-Lactamase Genes CTX-M, SHV, TEM and CIT-Type AmpCs in Enterobacteriaceae. PLoS One. 9(7).