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Ngày xuất bản: 24/11/2025
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DOI: https://doi.org/10.51298/vmj.v556i2.16196
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Tập 556 Số 2 (2025): Vietnam Medical Journal
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Le, S. H. ., Le-Nguyen, M.-P. ., Pham, U. V. ., & Nguyen, B.-L. T. . (2025). Antimicrobial effect of advanced platelet-rich fibrin plus against methicillin-susceptible and methicillin-resistant Staphylococcus aureus: An in vitro study. Tạp Chí Y học Việt Nam, 556(2). https://doi.org/10.51298/vmj.v556i2.16196

Antimicrobial effect of advanced platelet-rich fibrin plus against methicillin-susceptible and methicillin-resistant Staphylococcus aureus: An in vitro study

Le Son Hoang 1,, Le-Nguyen Minh-Phuc 1, Pham Uy Van 2, Nguyen Bich-Ly Thi 1
1 Department of Oral Surgery, Faculty of Dentistry, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
2 Department of Maxillofacial Surgery, Odonto-Maxillo-Facial Hospital in Ho Chi Minh City, Ho Chi Minh City, Vietnam

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Tóm tắt

Objectives: The virulence of methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) differs significantly; however, the antimicrobial effects of advanced platelet-rich fibrin plus (A-PRF+) on these subspecies remain unclear. This study aimed to evaluate the efficacy of A-PRF+ against MSSA and MRSA.


Methods: Fifteen male participants volunteered for this study. Solid and liquid forms of A-PRF+ were produced using the DUO Quattro centrifuge machine following the recommended protocol. The inoculum of MSSA and MRSA was prepared from reference samples obtained from the American Type Culture Collection. Both inocula were adjusted to a McFarland standard of 0.5. The antimicrobial effects of A-PRF+ against MSSA and MRSA were evaluated using disk diffusion assays, minimum inhibitory concentration (MIC) tests, and biofilm formation experiments.


Results: The disk diffusion assay demonstrated weak antimicrobial activity against both MSSA and MRSA, with inhibition zones measuring 0.68 ± 0.44 mm and 0.69 ± 0.35 mm, respectively. However, MIC testing revealed that A-PRF+ did not exhibit antimicrobial effects against either subspecies following dilution. Finally, A-PRF+ significantly reduced the biofilm-forming capacity of both MSSA and MRSA to approximately 70%.


Conclusion: A-PRF+ exhibited weak antimicrobial activity against both MSSA and MRSA in agar diffusion assays. Additionally, A-PRF+ reduced the biofilm-forming capacity of both MSSA and MRSA. However, no significant differences were detected in the antimicrobial effects of A-PRF+ between MSSA and MRSA.

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Từ khóa

platelet-rich fibrin; antimicrobial agent; in vitro techniques; methicillin-susceptible staphylococcus aureus; methicillin-resistant staphylococcus aureus

Tài liệu tham khảo

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