Article Sidebar
Views pdf: 28
DETERMINE NON-HAEMOLISIS STAPHYLOCOCCUS AUREUS FROM SPECIMENS CULTURED IN LABORATORY
Main Article Content
Abstract
Staphylococcus aureus is Gram-positive coccus, capable of causing infection in many organs in the body such as sepsis, skin and soft tissue infection, endocarditis, arthritis, meningitis, etc. When cultured on Blood agar medium, Staphylococcus aureus often causes beta hemolysis (complete hemolysis). This is one of the first characteristic features used to identify Staphylococcus aureus in the laboratory. Non-hemolytic strains are generally considered coagulase - negative Staphylococcus (CNS) and rarely cause infection. However, not all strains of Staphylococcus aureus are capable of causing beta hemolysis. A small number of them don’t cause hemolysis. Therefore, when culturing specimens in the laboratory, it is necessary to carry out the next identification steps when suspecting Staphylococcus to be pathogen, so as not to misdiagnosis in cases of non – hemolytic Staphylococcus aureus.
Article Details
Keywords
Staphylococcus aureus causes beta hemolysis, non – hemolytic Staphylococcus aureus
References
2. Lê Huy Chính (2012). Vi sinh vật Y học, NXB Y học, 133 – 141.
3. William R. Schwan, Michael H. Langhorne, Heather D. Ritchie et al. (2003). Loss of hemolysin expression in Staphylococcus aureus agr mutants correlates with selective survival during mixed infections in murine abscesses and wounds. FEMS Immunology & Medical Microbiology, 38 (1), 23 – 28.
4. Valerie E. Ryman, Felicia M. Kautz, and Steve C. Nickerson (2021). Case Study: Misdiagnosis of Nonhemolytic Staphylococcus aureus isolates from cases of Bovine Mastitis as Coagulase-Negative Staphylococci. Animals 2021, 11(2), 252.
5. Rebecca Buxton (2005). Blood Agar Plates and Hemolysis Protocols. American society for Microbilology, 30 September 2005.
6. Haifang Zhang, Yi Zheng, Huasheng Gao et al (2016). Identification and Characterization of Staphylococcus aureus strains with an Incomplete Hemolytic Phenotype. Front Cell Infect Microbiol, 2016; 6: 146.
7. Li – Juan Wang, Xin Yang, Su – Yun Qian (2020). Identification of hemolytic activity and hemolytic genes of Methicillin – resistant Staphylococcus aureus isolated from Chinese children. Chin Med J (Engl). 2020 Jan 5; 133(1): 88 – 90.
8. Stephen H. Gillespie, Peter M. Hawkey (2005). Principle and practice of clinical Bacteriology. John Wiley & Sons Ltd, 2nd edition, 84 – 85.
9. David P Kateete, Cyrus N Kimani, Fred A Katabaz et al. (2010). Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test. Annals of Clinical Microbiology and Antimicrobials, vol 9, Article number: 23 (2010), 153.
10. Connie R. Mahon, Donald C. Lehman, George Manuselis (2015). Text book of Diagnostic Microbiology. ELSEVIA, 5th edition, 323 – 324.