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Date Published: 07/09/2024
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DOI: https://doi.org/10.51298/vmj.v541i3.10918
Issue
Vol. 541 No. 3 (2024)
Section
Các bài báo
How to Cite
Nguyễn, H. P., & Phan, T. N. B. (2024). COMPARISON OF DIAGNOSTIC VALUE OF ANA-ELISA AND ANA IMMUNOFLUORESCENCE IN CONNECTIVE TISSUE DISEASES. Vietnam Medical Journal, 541(3). https://doi.org/10.51298/vmj.v541i3.10918

COMPARISON OF DIAGNOSTIC VALUE OF ANA-ELISA AND ANA IMMUNOFLUORESCENCE IN CONNECTIVE TISSUE DISEASES

Hoàng Phương Nguyễn, Thị Ngọc Bích Phan

Main Article Content

Abstract

Objective: Comparison of the diagnostic value of antinuclear antibody (ANA) testing using ELISA and indirect immunofluorescence (IIF) techniques in autoimmune diseases. Materials and methods: The study was conducted from October to December 2022. Sera from 59 patients with autoimmune diseases and 34 healthy controls were tested for ANA using ELISA (ANA-ELISA) and indirect immunofluorescence (ANA-IIF) techniques. Results: Among the 62 serum samples with ANA-IIF (+), the most common staining pattern was speckle (53.23%). The sensitivities of ANA-IIF and ANA-ELISA for autoimmune diseases were 97.22% and 91.76%, respectively, and for SLE were 88.14% and 86.44%, respectively. The specificity of ANA-ELISA for autoimmune diseases is 88.24%, higher than ANA-IIF at 70.59%. The false positive rate of ANA-IIF in the control group was 17.65% compared to 0% of ANA-ELISA. Conclusions: ANA-ELISA has lower sensitivity but higher specificity than ANA-IIF in screening and diagnosing autoimmune diseases.

Article Details

Keywords

antinuclear antibody, ELISA, indirect immunofluorescence.

References

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