NGHIÊN CỨU SƠ BỘ BIẾN THỂ SỐ LƯỢNG BẢN SAO DNA TY THỂ Ở BỆNH NHÂN UNG THƯ VÚ VIỆT NAM

Abstract

Background: Changes in mitochondrial DNA copy number (mtDNA-CN) in various tumor types have been linked to an increased risk of cancers. However, studies on their association with breast cancer in Vietnamese patients remain to be determined. In this pilot study, we investigated mtDNA-CN variation in paired normal and cancerous breast tissues and compared their differences. Methods: 12 paired tissues from adjacent normal and tumor sites from the same breast cancer patients were utilized for the study. Total genomic DNA was isolated and changes in mtDNA-CN were measured by relative quantification using the SYBR green based quantitative Real- time PCR method. Results: The R2 correlation for each standard curve was ≥0.98. The 12 individuals, averaging 45.83 years old (± 9.73), were identified with early-stage invasive or locally advanced breast cancer, some with no lymph node involvement (N0) and others with (N1). Tumors, classified as grade 2 or higher, with sizes ranging from 3 to 45 mm. Our results revealed a significant reduction in mtDNA-CN in cancerous breast tissues compared with the normal ones (p=0.0226). Conclusions: Overall, our study reports a comparison of mtDNA-CN in paired normal and cancerous breast tissues and suggests that decreased mtDNA content in breast cancer may have diagnostic value for the disease