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DETECTION OF IGH GENE REARRANGEMENTS IN MULTIPLE MYELOMA PATIENTS AT BLOOD TRANSFUSION HEMATOLOGY HOSPITAL
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Abstract
Objectives: Detection of IgH gene rearrangements in Vietnamese multiple myeloma patients using PCR technique. Subjects and methods: A prospective study was conducted on 43 patients with newly diagnosis of multiple myeloma at Blood Transfusion Hematology Hospital from June 2019 to June 2021. We detected IgH gene rearrangements using Multiplex PCR technique. Results: The frequency of IgH gene rearrangements were detected in 74.4% of patients using Vh (FR1) primers, the rate of detection were consecutively 95.3% and 97.7% when we combine Vh (FR1/2) primers and Vh (FR1/2/3) primers. Conclusions: By combining three primers set were designed for three FR regions, we could detect the IgH gene rearrangements in the majority of myeloma patients.
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Keywords
IgH gene rearrengement, multiple myeloma, FR region
References
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