SURVEY OF MICROORGANISMS IN ENDODONTIC-PERIODONTAL LESIONS ON SINGLE-ROOTED TEETH BEFORE AND AFTER TREATMENT
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Abstract
Objective: The study aims to investigate the microbiological changes in endo-periodontal lesions in single-rooted teeth before and after non-surgical endodontic and periodontal treatment. Materials and Methods: The study was conducted on 15 single-rooted teeth with endo-periodontal lesions treated at the Faculty of Odonto-Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City. Microbiological and clinical indices before and after treatment were recorded and compared. Microbiological samples were collected from the root canal and periodontal pockets, and then analyzed using real-time PCR. Results: The bacterial load in the root canal and periodontal pockets significantly decreased after treatment compared to before treatment. For Porphyromonas gingivalis (Pg), the median bacterial count in the root canal decreased from 62 Log10 copies/ml to 57 Log10 copies/ml (p = 0.043), while in the periodontal pockets, it decreased from 63 Log10 copies/ml to 50 Log10 copies/ml (p = 0.038). Similarly, Treponema denticola (Td) and Fusobacterium nucleatum (Fn) also showed significant reductions after treatment. Conclusion: Non-surgical endodontic and periodontal treatment significantly reduced the bacterial load in endo-periodontal lesions, contributing to the clinical improvement of patients. However, the reduction was not uniformly observed across all bacterial species.
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Keywords
endo-periodontal lesions, endodontic treatment, bacteria, real-time PCR
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