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Date Published: 19/09/2025
Abstract Views: 85
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DOI: https://doi.org/10.51298/vmj.v553i3.15479
Issue
Vol. 553 No. 3 (2025)
Section
Các bài báo
How to Cite
Lê, X. Ái, Trần, T. H. V., Văn, T. T. T., Lê, Đức V., & Huỳnh, M. T. (2025). PROTOCOL DEVELOPMENT AND CONCORDANCE ASSESSMENT OF BROTH MICRODILUTION VERSUS AUTOMATED ANTIFUNGAL SUSCEPTIBILITY TESTING IN CANDIDA SPP. Vietnam Medical Journal, 553(3). https://doi.org/10.51298/vmj.v553i3.15479

PROTOCOL DEVELOPMENT AND CONCORDANCE ASSESSMENT OF BROTH MICRODILUTION VERSUS AUTOMATED ANTIFUNGAL SUSCEPTIBILITY TESTING IN CANDIDA SPP

Xuân Ái Lê, Thị Huệ Vân Trần, Thị Thanh Thủy Văn, Đức Vinh Lê, Minh Tuấn Huỳnh

Main Article Content

Abstract

Background: Invasive Candida spp. infections are a common cause of nosocomial infections, particularly in intensive care units (ICUs), with high mortality rates and significant treatment costs. Antifungal susceptibility testing (AFST) to determine the minimum inhibitory concentration (MIC) and resistance category (RIS) is an essential tool for selecting effective treatment regimens. Standard broth microdilution methods are rarely utilized in Vietnam due to technical complexity and high cost, while automated systems such as Vitek-2, although effective, are unsuitable for small- to medium-sized healthcare facilities because of technical requirements, specialized equipment, and expense. The Sensititre YeastOne (SYO) commercial broth microdilution kit is designed to simplify procedures and eliminate the need for dedicated equipment. This study was conducted to establish a SYO-based testing protocol for Candida spp. and propose a suitable method for laboratories without access to automated systems. Objective: To develop a standardized SYO broth microdilution protocol and evaluate its concordance with the Vitek-2 automated system in testing Candida spp.. Methods: Internal organ-derived Candida spp. isolates were collected from University Medical Center Ho Chi Minh City. Relevant data were analyzed to formulate the SYO testing procedure. Results were compared with those from the Vitek-2 Compact system. Results: A successful SYO antifungal susceptibility testing protocol was developed. Specifically: (i) SYO MIC results demonstrated high concordance with Vitek-2 results (Essential Agreement [EA]: 100% for Caspofungin, 95.6% for Fluconazole, ≥ 91% for other antifungals); (ii) SYO successfully generated MIC values for all 70 Candida spp. isolates, indicating strong reliability and feasibility. Conclusion: SYO shows a high level of concordance with Vitek-2 and is well-suited for implementation in laboratories without automated systems, supporting the treatment of invasive Candida spp. infections.

Article Details

Keywords

Candida, antifungal susceptibility testing, Sensititre YeastOne (SYO

References

Pappas PG, Lionakis MS, Arendrup MC, et al. Invasive candidiasis. Nature Reviews Disease Primers. 2018;4(1):1-20.
2. Kotey FC, Dayie NT, Tetteh-Uarcoo PB, et al. Candida bloodstream infections: changes in epidemiology and increase in drug resistance. Infectious Diseases: Research and Treatment. 2021;14:11786337211026927.
3. Clinical Laboratory Standard Institute. Verification of Commercial Microbial Identification and Antimicrobial Susceptibility Testing Systems. Clinical and Laboratory Standards Institute; 2015.
4. Delgado R, Tibau XA. Why Cohen's Kappa should be avoided as performance measure in classification. PLoS One. 2019;14(9):e0222916. doi: 10.1371/journal.pone.0222916. PubMed PMID: 31557204; PubMed Central PMCID: PMCPMC6762152.
5. Wong KY, Gardam D, Boan P. Comparison of Vitek 2 YS08 with Sensititre YeastOne for Candida susceptibility testing. Pathology. 2019 Oct;51(6): 668-669. doi: 10.1016/j.pathol.2019 .05.005. PubMed PMID: 31470992.
6. Cuenca-Estrella M, Gomez-Lopez A, Alastruey-Izquierdo A, et al. Comparison of the Vitek 2 antifungal susceptibility system with the clinical and laboratory standards institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) Broth Microdilution Reference Methods and with the Sensititre YeastOne and Etest techniques for in vitro detection of antifungal resistance in yeast isolates. Journal of clinical microbiology. 2010;48(5):1782-1786.
7. Siqueira RA, Doi AM, de Petrus Crossara PP, et al. Evaluation of two commercial methods for the susceptibility testing of Candida species: Vitek 2((R)) and Sensititre YeastOne((R)). Rev Iberoam Micol. 2018 Apr-Jun;35(2):83-87. doi: 10.1016/ j.riam.2017.11.001. PubMed PMID: 29580699.
8. Pea F, Lewis RE. Overview of antifungal dosing in invasive candidiasis. J Antimicrob Chemother. 2018 Jan 1;73(suppl_1):i33-i43. doi: 10.1093/ jac/dkx447. PubMed PMID: 29304210.