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RESEARCH ON THE RELATIVE BENEFITS OF GERM TUBE TEST AND RAPID YEAST PLUS AGAINST CHROMAGAR CANDIDA IN IDENTIFYING CANDIDA SP.
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Abstract
Objective: To determine the diagnostic value of the germ tube test and CHROMagar Candida medium in the identification of Candida sp. Methods: A descriptive cross-sectional study was conducted on 89 Candida spp. isolates from clinical specimens collected at the Microbiology Department - Thong Nhat General Hospital, Dong Nai (10/2023-08/2024). Each isolate was simultaneously tested using the germ tube test, CHROMagar Candida, and the RapID Yeast Plus system (used as the reference method). Results: Among 89 isolates, C. albicans accounted for 67,4%; C. tropicalis 22,5%; C. krusei 5,6%; and C. glabrata 4,5%. The identification of C. albicans by both the germ tube test and CHROMagar showed high diagnostic values: sensitivity 98,3%; specificity 100%; positive predictive value (PPV) 100%; negative predictive value (NPV) 96,6%; and accuracy 98,9%. CHROMagar also provided high performance in the preliminary identification of non-albicans Candida species, with accuracy >93%. Agreement among methods was very high (Kappa > 0,9). Conclusion: The germ tube test is a simple, rapid (2-4 hours), and highly accurate method for identifying C. albicans. Meanwhile, CHROMagar Candida allows effective preliminary identification of common Candida spp. within 24 hours. This study contributes additional data on the advantages and limitations of each method, helping laboratories choose suitable approaches for rapid and accurate species identification.
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Keywords
germ tube, Rapid Yeast Plus, CHROMagar Candida, Candida
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