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RESEARCH ON THE DISTRIBUTION OF HUMAN PAPILLOMAVIRUS (HPV) IN TONSILLAR SQUAMOUS CELL CARCINOMA DURING 2018 – 2022
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Abstract
Purpose: The study was conducted to determine the types and prevalence of HPV infection in patients with tonsil squamous cell carcinoma. Materials and methods: Cross-sectional descriptive study on 167 biopsy tissue samples of patients diagnosed with tonsil squamous cell carcinoma at Tan Trieu K Hospital from 2018-2022 using Nested-PCR technique and Sanger gene sequencing method. Results: 83/167 specimens were identified as HPV+, accounting for 49.7%. 5 HPV types were detected: 65/83 (78.3%) cases of HPV16, 2 cases of HPV58 and 1 case of HPV18 (all high-risk HPV) and 14/83 (16.9%) cases of HPV44, 1 case of HPV26 (all low-risk HPV). The proportion of female patients with this cancer having HPV+ (88.9%) was statistically higher than that of male patients (45%). The HPV+ rate was highest in stage III (64.7%) and decreased in stage IV, with the highest number of HPV- (48/81) and there was a statistically significant difference in the distribution of disease stages between the HPV+ and HPV- groups. Conclusion: Through the study of 167 patients with tonsil squamous cell carcinoma, we detected 83 cases of HPV+, accounting for 49.7%. Gene sequencing detected five types HR - HPV16, 18, 58 and LR - HPV44, 26, accounting for 78.3%, 2.4%, 1.2% and 16.9%, 1.2%, respectively. The proportion of female patients with this cancer having HPV+ (88.9%) was higher than that of male patients (45%) with statistical significance with p < 0.001. The HPV+ rate was highest in stage III (64.7%) and decreased in stage IV, with the highest number of HPV- (59.3%) and the difference was statistically significant
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Keywords
tonsillar squamous cell carcinoma, HPV
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