KHẢO SÁT TỶ LỆ VÀ TÍNH ĐỀ KHÁNG KHÁNG SINH CÁC CHỦNG ESCHERICHIA COLI MANG GEN SINH ĐỘC TỐ GÂY TIÊU CHẢY CẤP Ở TRẺ EM

Abstract

Background: Escherichia coli (E. coli) is one of the important agents that cause diarrhea in children. However, with conventional culture methods, it is not possible to distinguish pathogenic E. coli from resident E. coli. Therefore, Multiplex Real-time PCR to identify the virulent genes of E. coli is very important in the investigation of the etiology of the disease. This study investigates the prevalence and antibiotic resistance patterns of diarrheagenic E. coli (DEC) that were isolated from infectious diarrhea samples of pediatric patients. Objective: Identify the rate of E. coli diarrheal species in children include: EAEC, ETEC, EPEC, EHEC, and E. coli O157 at Hoan My Da Lat Hospital. Identify the antibiotic resistance, the frequency of multidrug resistance (MDR) and the extended spectrum-β-lactamase (ESBL) of isolated DEC. Subjects and methods: The study examined 81 strains of Escherichia coli isolated from stool samples of under 5 years old children with acute diarrhea at Hoan My Da Lat Hospital. Using Multiplex Real-time PCR technique to determine the incidence of E. coli diarrheal types and diffusion techniques agar to determine the antibiotic resistance, MDR, ESBL. Results: Among 81 E. coli isolated, 9 cases (11,1%) were identified as diarrheagenic E. coli. EAEC was the predominant (4 cases; 4,9%), followed by EPEC (2 cases; 2,5%) and EHEC (2 cases; 2,5%), ETEC (1 cases; 1,2%). However, Co-infection and E. coli O157 were not detected. Extended-spectrum β-lactamase (ESBL) was detected in 4 isolated (44,4%). Multidrug-resistance (MDR) was found in 77,8% of isolated DEC. The rate of resistance to Trimethoprim-Sulphamethoxazole (SXT) was the highest (88.9%), the rate of resistance to Cephalosporins was 77,8%; while 100% strains were susceptible to Amikacin, Imipenem and Meropenem. Conclusions: Multiplex Real-time PCR is necessary to distinguish pathogenic E. coli from resident E. coli to choose an appropriate initial antibiotic in the treatment.