ẢNH HƯỞNG IN VITRO CỦA NANO BERBERINE LÊN SỰ CO GEL COLLAGEN CỦA NGUYÊN BÀO SỢI NƯỚU NGƯỜI

Abstract

Objectives: In order to provide a scientific basis for the application of BBr in the healing of gingival-oral tissues, we carried out a research survey with the aim of evaluating the in vitro effects of Nano BBr on some biological properties collagen gel contraction of hGF. Materials and methods: The collagen gel contraction test is performed by implanting cells into type I collagen to form a disc-shaped gel. Type I collagen is easy to polymerize to create a fibrous network that mimics the collagen framework in the connective tissue. Nano Berberine 2% was diluted 1, 1/10, 1/102, 1/103, 1/104 to the hGF culture plates that had been incubated for 24h. Use Nano BBr at a proven non-toxic concentration to evaluate the biological properties of hGF collagen gel contraction in cultures containing Nano BBr. Cell culture and tests were carried out at the Laboratory of Biomedical Materials and Tissue Engineering, University of Natural Sciences, Viet Nam National University, Ho Chi Minh City. Results: The obtained results showed that collagen gel contraction was recorded in the medium standard treatment. The standard treatments without nutritional supplementation and the experimental treatments did not record collagen gel contraction. However, no statistically significant difference in collagen gel contraction was observed when all 3 treatments were compared with 0 day. Conclusions: Collagen contraction was recorded in the medium standard treatment. The standard treatments without nutritional supplementation and the experimental treatments did not record collagen gel contraction.