ỨNG DỤNG KỸ THUẬT REAL TIME PCR PHÁT HIỆN KÝ SINH TRÙNG SỐT RÉT Ở BỆNH PHẨM TRÊN GIẤY THẤM VÀ BỆNH PHẨM BẢO QUẢN BẰNG EDTA

Abstract

Introduction: Malaria is an infection diease caused by Plasmodium species. through the malaria vector, the Anopheles mosquito, in Vietnam this disease is mainly caused by Plasmodium falciparum and Plasmodium vivax, which mainly circulates throughout the year in the forest, mountainous and coastal areas where diagnostic methods for detecting this disease are often not easily accessible. There are many detection methods to find malaria parasites but most of them are not highly sensitive, require technician experience and require a large amount of blood. The application of dried blood spot in malaria parasite detection by molecular assay Real time PCR shows the efficiency in diagnostic, cost and convenient storage – transportation capable of practical application for epidemiological investigation and epidemic localization. Objective: Evaluation of the effectiveness of the Real time PCR assay in detecting malaria parasite on Whatmann 3MM dried blood spot compared to blood sample in EDTA anticoagulation. Materials and method: The study described a series of cases conducted on 19 samples of Whatmann 3MM and 19 blood samples with EDTA collected from malaria parasite culture, and evaluated the technical effectiveness on 10 capillary blotting paper samples of patients suspected of from malaria parasite infection collected at the Institute of Malaria – Parasitology – Insects. HCMC from 07/2022-10/2022. Result: There was no difference in the results of malaria parasite detection in both specimens collected from malaria parasite culture, the detectable malaria parasite density was 3 parasitesml of blood by Real time PCR.