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Date Published: 12/04/2023
Abstract Views: 146
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DOI: https://doi.org/10.51298/vmj.v525i1A.5012
Issue
Vol. 525 No. 1A (2023)
Section
Các bài báo
How to Cite
Phạm , N. L. ., Nguyễn, H. L. T. ., Trương , V. Đạt ., Trương, M. N. ., & Lê, M. T. . (2023). STIMULTANEOUS DETERMINATION OF NARINGIN AND HESPERIDIN IN YOUNG POMELO BY CAPILLARY ELECTROPHORESIS METHOD. Vietnam Medical Journal, 525(1A). https://doi.org/10.51298/vmj.v525i1A.5012

STIMULTANEOUS DETERMINATION OF NARINGIN AND HESPERIDIN IN YOUNG POMELO BY CAPILLARY ELECTROPHORESIS METHOD

Ngọc Liên Phạm 1, Hữu Lạc Thủy Nguyễn1, Văn Đạt Trương 1, Minh Nhựt Trương1, Minh Trí Lê1,
1 University of Medicine and Pharmacy at Ho Chi Minh City

Main Article Content

Abstract

Objectives: To develop an analytical methodology to simultaneously determine naringin and hesperidin in young pomelo powder. Subjects and methods: Naringin and hesperidin in young pomelo powder were determined by CE method. Results: The suitable conditions for separation of naringin and hesperidin were obtained using borate buffer 50 mM, pH 10.0; injection condition: 50 mbar per 10 seconds; column temperature 25 oC; voltage 20 kV and 254 nm PDA detector. The process was validated the system suitsability; selectivity; linearity range naringin: 10.0 – 500.0 (ppm), y = 0.0201x; r = 0.9993 and hesperidin: 5.0 – 50.0 (ppm), y = 0.0191x; r = 0.9996; precision of both compounds with RSD < 5.3 %; and accuracy with recovery ratio in 80 – 110 (%). Conclusion: The process could be applied to determine the content of naringin and hesperidin in young pomelo powder.

Article Details

Keywords

naringin, hesperidin, young pomelo powder, CE.

References

1. AOAC International. AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals. Section 3.4. 2002:17-22.
2. Ani PN, Abel HC. Nutrient, phytochemical, and antinutrient composition of Citrus maxima fruit juice and peel extract. Food Sci Nutr. 2018;6(3):653-658.
3. Đỗ Tất Lợi. Những cây thuốc và vị thuốc Việt Nam. Nhà xuất bản Y học. 2006.
4. ICH Harmonised Tripartite Guideline. Validation of analytical procedures: text and methodology 2005:1-13.
5. Tocmo R, Pena-Fronteras J, Calumba KF, Mendoza M, Johnson JJ. Valorization of pomelo (Citrus grandis Osbeck) peel: A review of current utilization, phytochemistry, bioactivities, and mechanisms of action. Compr Rev Food 2020;19(4):1969-2012.
6. Wu T, Guan Y, Ye J. Determination of flavonoids and ascorbic acid in grapefruit peel and juice by capillary electrophoresis with electrochemical detection. Food Chem. 2007;100(4):1573-1579.
7. Yu EA, Kim GS, Lee JE, et al. Flavonoid profiles of immature and mature fruit tissues of Citrus grandis Osbeck (Dangyuja) and overall contribution to the antioxidant effect. Biomed Chromatogr. 2015;29(7):1131.
8. Zhong H, Yao Q, Breadmore MC, Li Y, Lu Y. Analysis of flavonoids by capillary zone electrophoresis with electrokinetic supercharging. Analyst. 2011;136(21):4486-4491.