COMPARE THE ACCURACY OF THE ASSESSMENT OF PLASMA INDICES BY THE VISUAL MEASUREMENTS USING COLOR METRIC WITH THE AUTOMATIC ANALYSIS METHOD ON BIO-IMMUNOASSAY MACHINE
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Abstract
Introduction: Serological index assessed by visual senses, though simple, inexpensive, used in many laboratories, but very subjective and the accuracy has not been verified. Determining the accuracy of the visual and instrumental evaluation is essential for the quality management of the pre-analytical samples. Objective: Determination of the accuracy of the assessment of serum indices at all concentrations by the visual measurements using color metric with the automatic analysis method on ARCHITECT CI8200. Subjects and methods: Serum samples were collected from 07/2020 - 11/2020 at the laboratory, Nguyen Tri Phuong hospital, assessed for three serum indices by two methods of visual sensory and machine. The results were independently recorded and processed to evaluate the accuracy of the visual sensory method compared with automatic analysis by the machine. Results: 420 samples, of which 121 samples had no serum index, 260 samples had 1 serum index and 39 samples had more than one serum index. For samples with only one H-index, I-index, L-index, the accuracy when distinguishing between the 0-1234 level between the visual sensory method on the automatic system is 0.87; 0.72; 0.84. For H-index, accuracy between the two methods is at 0-1 concentration levels; 1-2, 2-3, 3-4 are 0.86 respectively; 0.76; 0.59; 0.53. For index I, accuracy between the two methods is at 0-1 concentration levels; 1-2, 2-3, 3-4 are 0.72 respectively; 0.58; 0.50; 0.40. For the L index, the accuracy between the two methods is at 0-1 concentrations; 1-2, 2-3, 3-4 are 0.84 respectively; 0.57; 0.33 0.50. For samples with two or more indices due to the interaction of the indices in the same sample, the accuracy between the two methods cannot be determined. Conclusions: The visual measurements using color metric has good accuracy in evaluating samples with or without serum indices. At different concentrations in the same index, the method of visual assessment less accurate in the classification.
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Keywords
Serum indices, hemolysis, icteric, lipemic
References
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