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Date Published: 27/06/2023
Abstract Views: 131
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DOI: https://doi.org/10.51298/vmj.v527i1B.5758
Issue
Vol. 527 No. 1B (2023)
Section
Các bài báo
How to Cite
Nguyễn, Q. T., Nguyễn, M. T., Mai, H. N., & Vũ, T. T. (2023). SOLUBILITY PREDICTION AND VECTOR DESIGN FOR THE EXPRESSION OF HUMAN INTERLEUKIN-2 IN ESCHERICHIA COLI. Vietnam Medical Journal, 527(1B). https://doi.org/10.51298/vmj.v527i1B.5758

SOLUBILITY PREDICTION AND VECTOR DESIGN FOR THE EXPRESSION OF HUMAN INTERLEUKIN-2 IN ESCHERICHIA COLI

Quốc Thái Nguyễn1,, Minh Thư Nguyễn1, Huỳnh Như Mai1, Thanh Thảo Vũ1
1 University of Medicine and Pharmacy at Ho Chi Minh City

Main Article Content

Abstract

Background: Interleukin-2 (IL-2) plays an important role in T cell formation, development, differentiation, and homeostasis; it has been approved by the FDA for the treatments of metastatic renal cell carcinoma and metastatic melanoma. Objectives: This study aims to predict the solubility of human IL-2, from which a recombinant plasmid to express IL-2 as soluble protein in Escherichia coli can be designed. Methods: Three web-based tools including PSOLart, NetSolP and SoDoPE were used to predict the solubility of IL-2 alone and when being fused with tags. Results: The solubility of IL-2 was predicted as average to poor by all three tools. NetSolP indicated that the expressibility of IL-2 in E. coli is low; the solubility can be improved with tags MBP and SUMO as suggested by SoDoPE. Conclusions: The study has predicted the solubility of human IL-2 and designed a recombinant plasmid in which IL-2 is fused with SUMO and condons optimized for expression in E. coli

Article Details

Keywords

interleukin-2, solubility, SUMO tag

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