XÂY DỰNG QUY TRÌNH CHIẾT CAO CHUẨN HÓA KIỂM SOÁT HÀM LƯỢNG CURCUMINOID TỪ THÂN RỄ NGHỆ VÀNG (RHIZOMA CURCUMAE LONGA)

Abstract

Background: Curcuminoid, the main compound that gives turmeric its yellow color and biological activity, has many medical applications. This study focused on investigating the process of extracting curcuminoids from turmeric rhizomes with a low-toxic, environment-friendly solvent and creating a highly standardized extract with controlled curcuminoid content. This could be a promising direction for a potential pharmaceutical industry approach to creating products that protect human health. Objectives: Develop a standardized and controlled extraction process of 100 grams of curcuminoid from turmeric rhizomes. Materials and methods: Raw yellow turmeric rhizomes were collected in U Minh Thuong district, Kien Giang province. They were then dried, pounded into powder, and kept to meet the requirements for testing medicinal herbs following Vietnam Pharmacopoeia standards before usage. The sample was first alkalized, then filtered to collect the solution, and finally acidified to a pH level that would precipitate curcuminoid. A highly standardized extract of turmeric with controlled curcuminoid content was determined by high-performance liquid chromatography coupled with a photodiode array detector (HPLC/PDA). Results: The extraction conditions were obtained, included the following: NaOH, pH = 13, tartaric acid, pH acidification of alkaline solution = 3, precipitation time was 4 hours. From 2 Kg of turmeric powder meeting the Vietnam Pharmacopoeia standards, 100 grams of turmeric standardized extract containing 27.5% curcuminoid was obtained. Conclusion: The study successfully developed a highly standardized extraction process with controlled curcuminoid content from the scale of 2 Kg of yellow turmeric and can be performed on a pilot scale.