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Date Published: 07/08/2023
Abstract Views: 204
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DOI: https://doi.org/10.51298/vmj.v528i2.6085
Issue
Vol. 528 No. 2 (2023)
Section
Các bài báo
How to Cite
Nguyễn, V. P., Bùi, T. B. L., Nguyễn, V. P., Hoàng, V. T., & Trần, V. T. (2023). TETRA-PRIMER ARMS PCR OPTIMIZATION TO DETECT SINGLE NUCLEOTIDE POLYMORPHISM RS7228049 OF SOCS6 GENE IN VIETNAMESE. Vietnam Medical Journal, 528(2). https://doi.org/10.51298/vmj.v528i2.6085

TETRA-PRIMER ARMS PCR OPTIMIZATION TO DETECT SINGLE NUCLEOTIDE POLYMORPHISM RS7228049 OF SOCS6 GENE IN VIETNAMESE

Việt Phương Nguyễn1,, Thị Bích Loan Bùi2, Vĩnh Phước Nguyễn2, Văn Tổng Hoàng3, Viết Tiến Trần1
1 103 Military Hospital
2 University of Science - Vietnam National University, Hanoi
3 Military Medical Research Institute

Main Article Content

Abstract

Objectives: To establish a protocol to detect single nucleotide polymorphism (SNP) rs7228049 of SOCS6 gene in 120 healthy Vietnamese indivuduals by using Tetra-primer ARMS PCR method. Subjects   and research methods: The SOCS6 SNP rs7228049 was detected by using T-ARMS PCR method, compared with Sanger sequencing results in 120 healthy Vietnamese indivuduals. Results: The homozygotes AA, GG and heterozygote GA genotypes of SOCS6 SNP rs7228049 were detected by T-ARMS PCR. In 120 healthy Vietnamese people, the frequency of alleles G and A were 63.3% and 36.7%; the frequency genotype GG was 50.0%, GA was 26.7% and AA was 23.3%, respectively. Conclusion: T-ARMS PCR has been designed, and optimized to genotype the SOCS6 SNP rs2062345 and initially screened the frequency of alleles and genotypes in the Vietnamese population.

Article Details

Keywords

Single nucleotide polymorphism (SNP), Tetra-primer ARMS PCR, SOCS6 gene

References

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