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Date Published: 07/08/2023
Abstract Views: 160
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DOI: https://doi.org/10.51298/vmj.v528i2.6133
Issue
Vol. 528 No. 2 (2023)
Section
Các bài báo
How to Cite
Đinh, T. T., Nguyễn, C. T., Nguyễn, P. T., Ngô, T. T., & Lê, H. S. (2023). ESTABLISHING PROTOCOL OF EXPRESSION OF RECOMBINANT BR512 ENZYME AND ITS APPLICATION FOR THE DIAGNOSTICS OF HUMAN PAPILLOMAVIRUS TYPE 18 USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION. Vietnam Medical Journal, 528(2). https://doi.org/10.51298/vmj.v528i2.6133

ESTABLISHING PROTOCOL OF EXPRESSION OF RECOMBINANT BR512 ENZYME AND ITS APPLICATION FOR THE DIAGNOSTICS OF HUMAN PAPILLOMAVIRUS TYPE 18 USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION

Thị Thảo Đinh1, Cẩm Thạch Nguyễn1, Phú Thành Nguyễn1, Tất Trung Ngô1, Hữu Song Lê1
1 108 Military Central Hospital

Main Article Content

Abstract

Objective: Establishing protocol to produce recombinant Br512 enzyme and investigate its capability in LAMP assay to detect Human Papillomavirus type 18 (HPV18). Material and method: E. coli BL21(DE3) cells was transformed with pKAR2-Br512 encoding plasmid then induced with specific inducer (IPTG); the expressed Br512 enzyme was purified by affinity chromatography; its enzymatic activity was evaluated and compared with commercial Bst 2.0 polymerase (Biolab NewEngland) for identifying HPV18 based on LAMP technique. Result: The in-house Br512 enzyme was highly pure enough and retains its LAMP activity in comparable with that of  Bst 2.0 from New England Biolab; both products could sense HPV18 at 10 copies/reaction and with clear signals. Conclusion: The recombinant Br512 enzyme was in house produced successfully with relevant enzymatic activity for LAMP based assay to detect HPV18 at 10 copies/ reaction.

Article Details

Keywords

Br512 polymerase, Loop-mediated Isothermal Amplification, Human Papillomavirus type 18

References

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