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STUDY ON PREPARED PROCEDURE ROSMARINIC ACID STANDARDIZED EXTRACT FROM FOLIUM PERILLA FRUTESCENS COLLECTED IN KIEN GIANG PROVINCE
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Abstract
Background: Perilla frutescens (L.) Britt (Lamiaceae) is a medicinal plant with seVeral purposes that has been used for many years and is quite popular in Vietnam. In particular, rosmarinic acid is one of the primary phenolic components in perilla leaf, which has been inVestigated to haVe beneficial effects such as: lowering blood uric acid, anti-inflammatory, antioxidant, inhibiting cancer cell growth, and so on. SeVeral products are prepared from Perilla frutescens including powder, folium Perilla frutescens drink, and totality extract. HoweVer, the control of rosmarinic acid composition in folium Perilla frutescens extract
linked to anti-inflammatory and uric acid-lowering effects in folium Perilla frutescens extract has almost not been studied, decreasing the product quality, effectiVeness and safety. At the same time, this research serVes as the foundation for current in ViVo pharmacological studies as well as trials of deVeloping goods, functional foods, and pharmaceuticals to aid in treatment and enhance human health. Objectives: DeVelop a standardized extraction process to regulate the rosmarinic acid content from folium Perilla frutescens collected in Kien Giang proVince. Materials and methods: Folium Perilla frutescens collected in Kien Giang proVince met the Vietnamese Pharmacopoeia V testing standards for medicinal plants. We employed the cold soaking technique to find out the extraction conditions (extraction solVent, time, the number of extraction times, the amount of solVent) and the impurity remoVal procedure. High performance liquid chromatography coupled with a photodiode arrays detector (HPLC-PDA) was used to determine the controlled extract of rosmarinic acid. Results: The optimal extraction parameter include 700 alcohol – acetic acid, one time of extraction, and material-solVent ratio (1:30). From 1 kg of dried folium Perilla frutescens to obtain 160 g of controlled extract of rosmarinic acid with 3.6% of rosmarinic acid were determined by HPLC/PDA method. Conclusion: This study successfully deVeloped a standardized extraction procedure with controlled rosmarinic acid concentration from 1 kg dried leaVes of folium Perilla frutescens gathered in Kien Giang proVince. This suggested approach is suitable for the industrial-scale production of safe and effectiVe products
Article Details
Keywords
rosmarinic acid, standardized extraction, folium Perilla frutescens.
References
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