MỐI LIÊN QUAN GIỮA MỨC ĐỘ BIỂU HIỆN VÀ TÍNH ĐA HÌNH CỦA GEN PKLR VỚI TỔNG ĐƯƠNG LƯỢNG ĐỘC CỦA POLYCHLORINATED DIBENZOFURAN (PCDDs), POLYCHLORINATED DIBENZOFURAN (PCDFs) VÀ PCDD/PCDFs Ở NGƯỜI PHƠI NHIỄM DIOXIN CÓ NGUỒN GỐC TỪ CHẤT DA CAM

Abstract

Objectives: The study aims to evaluate the relationship between copy number variation, rs3020781 polymorphism PKLR gene expression levels and pyruvate kinase enzyme activity with the total toxic equivalent (TEQ) of PCDDs, PCDFs and PCDD/PCDFs in blood in people exposed to dioxin derived from Agent Orange. Methods: including 100 people exposed to dioxin derived from Agent Orange. Using the real-time PCR method to determine the mRNA expression levels and copy number variation of the PKLR gene; using Sanger gene sequencing method to determine genotype distribution at rs3020781 polymorphism and ELISA method to determine pyruvate kinase enzyme activity. Results: There was no association between copy number (unrounded) of the PKLR gene and TEQ of PCDD (Polychlorinated dibenzo-p-dioxin), PCDFs (Polychlorinated dibenzofuran) and PCDD/PCDFs. However, there was an association between TEQ of PCDD and PCDD/PCDFs with copy number groups (rounded) and genotypic distribution at rs3020781 polymorphism of PKLR gene, with p<0.05. The mRNA expression levels of the PKLR gene were moderately positively correlated with the TEQ of PCDD and PCDD/PCDFs, with the correlation coefficients r=0.52 and r=0.506, respectively, p<0.01. However, pyruvate kinase enzyme activity was moderately inversely correlated with TEQ of PCDD, PCDD/PCDFs, insignificantly with TEQ of PCDFs with the correlation coefficients: r=−0.514 and r=-0.518, and r=-0.293, respectively, p<0.01. Conclusion: exposure to dioxin derived from Agent Orange can affect the mRNA expression levels of the PKLR gene and pyruvate kinase enzyme activity.