ỨNG DỤNG KỸ THUẬT PCR ĐA MỒI TRONG CHẨN ĐOÁN CĂN NGUYÊN GÂY NHIỄM TRÙNG ĐƯỜNG HÔ HẤP VÀ MỐI TƯƠNG QUAN VỚI NHIỄM TRÙNG HÔ HẤP THỨ PHÁT TẠI BỆNH VIỆN ĐẠI HỌC Y DƯỢC THÀNH PHỐ HỒ CHÍ MINH

Abstract

Background: Lower respiratory tract infections are quite common diseases, acquired at different ages and can leave many harmful effects on health. It is essential to have a diagnostic test for the causative agent of the disease, thereby obtaining an appropriate and effective treatment regimen. Objective: Toinvestigate the etiology of lower respiratory tract infections and the correlation with secondary bacterial infections. Methods: Cross-sectional descriptive study. 82 patients were diagnosed with acute lower respiratory tract infections and treated at the University Medical Center in Ho Chi Minh City from 11/2021 to 01/2023. Patients were subjected to multiplex PCR tests using Respiratory panel assays panel 1 and panel 4 of Seegene and automated bacterial culture on the Vitek 2 compact system. Results: PCR positive for pathogens was 43.9%. The most common causative agent for bacteria is Haemophilus influenza (25%), with viruses being influenza B (13.9%). The risk of secondary infection increases 12 times when the primary infectious agent is a virus. Conclusion: Multiplex Real-time PCR helps to increase the ability to detect lower respiratory tract infections, especially viruses and intracellular bacteria, bacteria that are difficult to culture, thereby limiting the overuse of antibiotics or selection. more suitable antibiotics.