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THE EFFECTIVENESS OF SPERMATID INJECTION IN INFERTILITY TREATMENT FOR MEN WITH NON-OBSTRUCTIVE AZOOSPERMIA
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Abstract
Background and aim: About 1% of men suffer from azoospermia, a condition of having no sperm in the ejaculate. Non-obstructive azoospermia, which affects 60% of azoospermia patients, is the most severe form of male infertility and requires testicular sperm extraction for sperm retrieval. Despite a 50% success rate, if no mature sperm is found, NOA men have no choice to become biological fathers. To overcome this, researchers have attempted to use spermatid injection techniques, such as round spermatid injection (ROSI) or elongating/elongated spermatid injection (ELSI), to give hope for pregnancy. Spermatids are immature sperm that have completed the second meiotic division and contain a haploid genome as mature spermatozoa. This cohort study aimed to evaluate the effectiveness of ROSI and ELSI in cases where spermatozoa were unavailable. Method: Couples with male factor infertility (men diagnosed with NOA) underwent IVF cycles. The intervention group received ROSI and ELSI. The primary outcome measures were fertilization, embryo development, clinical pregnancy, and live birth rate. Results: There were sixty-six ROSI and five ELSI cycles. The overall fertilization rate with the formation of two pronuclei after ROSI and ELSI was 35.1% and 64.5%, respectively. The clinical pregnancy rate of ROSI was 1.3%, much lower than ELSI (37.5%). Normal pregnancy and live birth were obtained in both groups. Conclusion: ROSI and ELSI can be considered alternative methods of infertility treatment in cases where spermatozoa are unavailable
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Keywords
Round Spermatid Injection, Elongrated Spermatid Injection, Elongating Spermatid Injection, Non-Obstructive Azoospermia, ROSI, ELSI, NOA
References
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