MỐI LIÊN QUAN GIỮA NỒNG ĐỘ IgE, SỐ LƯỢNG BẠCH CẦU ÁI TOAN VÀ NHIỄM ẤU TRÙNG GIUN ĐŨA CHÓ Ở TRẺ EM THÀNH PHỐ HỒ CHÍ MINH

Abstract

Objective: Toxocara canis infection can occur in all ages, if it is not detected and treated promptly, it will mainly not go away on its own and can create a danger to human health, especially children. ELISA test to quantify specific IgG in serum is a widely used test in clinical practice to diagnose toxocariasis, however it does not distinguish between old and new infections, so some tests other have been made to be used to support in diagnosis. Our study evaluates the relationship between IgE and eosinophil levels and T.canis infection in children in Ho Chi Minh City in Vietnam. Methods: A cross-sectional study was conducted from March to April 2023 with a sample size of 986 children from 3 to 15 years old. Serum samples were collected for investigating IgE concentration, eosinophils and anti-Toxocara immunoglobulin G (IgG) by using enzyme-linked immunosorbent assay (ELISA). ELISA testing by using the American-made AccuDiag™ Toxocara IgG ELISA Kit. Values of optical density greater than 0.3 according to the serological ELISA test were considered positive. Results: The seropositivity rate for Toxocara canis in children in Ho Chi Minh City, Vietnam is 14.2%. There was a statistically significant difference in the average values of MCH, MCV, eosinophils, SGOT (AST) and IgE between the two groups of Toxocara canis ELISA positive and negative, p < 0.05. In the positive ELISA group, optical density 0,3≤ OD<0,5 prevailed. There is  a positive association between IgE concentration and eosinophil count. Conclusion: The majority of children with ELISA positive for Toxocara canis in the study had antibody levels of 0.3≤ OD<0.5. There was a significant association between IgE concentration and eosinophil count. The positive association between IgE concentration and eosinophil count suggests these two parameters are valuable markers for the evaluation of the toxocariasis.